Determination of Brain Catecholamines by High Performance Liquid Chromatography with mewly developed Electrochemical Detector
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This paper confirms the usefulness of electrochemical detector (ED) for liquid chromatography in determining picogram amounts of catecholamines in mouse and rat brain. The extraction procedure was based on modifications of Kissinger's method and of Anton and Sayre, in which catecholamines were adsorbed on aluminum oxide and eluted with perchloric acid. Thedetector operates down to the 0.2-0.4ng range. Norepinephrine (NE) Ievels were 431±18ng/g in mouse brain and 426±19ng/g in the rat. Dopamine (DM) levels were 1,088±66ng/g in mouse brain and 787±33ng/g in the rat. The authors have-fouad significant changes in DM levels in rat whole brain depending on the method of sacrifice. Levels of NE in the forebrain after electrolytic destruction of the locus coeruleus were significantly decreased (24.2%) from that of control.
- Japan Society of Clinical Chemistryの論文
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- Determination of Brain Catecholamines by High Performance Liquid Chromatography with mewly developed Electrochemical Detector