Enzymatic Determination of Serum Creatinine Using Creatinine de minase

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概要

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• An enzymatic determination of serum creatinine using creatinine deiminase (EC 3.5.4.-) and glutamatedehydrogenase (EC 1.4.1.2) is described. The principle of this method is as follows;creatinine is degradated by creatinine deiminase to produce ammonia and N-methylhydantoin, and the ammonia produced is coupled with glutamate dehydrogenase. The change in absorbance at 340nm is mesured.<BR>The correlation coefflcient between the present method and Folin method was 0.997 and regression line was y=1.0x-0.26 (y=present method In this method). serum creatinine is determined without deproteinization.

• Japan Society of Clinical Chemistryの論文

著者

• 古川 一郎

  大阪大学中央臨床検査部

• 中西 千恵子

  大阪大学中央臨床検査部

• 林 長蔵

  大阪大学中央臨床検査部

関連論文

• Enzymatic Determination of Serum Creatinine Using Creatinine de minase
• Enzyme immunoassays for steroid hormomes

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