Microdetermination of Nicotine and Its Metabolites in Biological Fluid:1. A Fundamental Investigation on Gas Chromatography equipped with High Sensitive Nitrogen Detector

概要

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A fundamental study on determinations of minute nicotine and its metabolites has been performed with gas chromatography equipped with high sensitive nitrogen detector.<BR>Hitachi flame thermionic detector (FTD) is very convenient and has a much more specific sensitivity to nicotine when a hydrogen flow rate is reduced. For a stationary liquid phase of column packings, 10% Apiezon L+10% KOH is recommended. When hydrogen flow rate was reduced under 2ml min. a hydrocarbon peak and solvent peak were almost disappeared and a base line became very stable and less noisy. Programing analysis at the temperature from 190°C to 240°C was possible even with a single column.<BR>The coefficient of variation (C. V.) was under 5% in the internal standard method, and the detection limit of this method was about 100pg. as for nicotine.
Japan Society of Clinical Chemistryの論文