Urinary Acidic Glycosaminoglycans in Patients with Systemic Scleroderma and its Changes by Clinical Improvement Treated with a Few Medicaments
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Acidic glycosaminoglycans (AGG) in the urinary specimens of patients with systemic scleroderma, were prepared by the modified method of Antonopoulos <I>et al</I>. It was found that Dowex 1×2, column chromatography showed existence of chondroitin sulfate (chs) B and heparan sulfate (HS) besides chs A and C. As for HS, one or more bands were detected by the electrophoretic separation on the cellulose acetate strip by the method of Seno <I>et al</I>. The identification was made by elimination after the enzymatic digestion with chondrotinase (chase) ABC and AC.<BR>In the course of AGG preparation, the Pro-OH insoluble fraction was found to be much increased as compared with the urinary specimen of the normal individual. The fraction was applied to Dowex 1×2, column chromatography, and resulted to reveal chs B and its variant. As a result of paper chromatographic separation of the unsaturated disaccharides after digestion with chase ABC and AC, it was observed that approx. 50% of the sample remained at the original portion and two spots were visualized between the site of Δdi6S and the original portion. The evidences of lower uronic acid content, positive Molisch reaction and the resistence for digestion with condroitinases, may lead to existence of keratan sulfate in the Pro-OH insoluble fraction.<BR>A few kinds of medicaments <I>i.e</I>. asiaticoside (Madecassol<SUP>®</SUP>), D-thyroxine and the human placenta preparation (PLP<SUP>®</SUP>), were used for the treatment. It was found that comparative AGG-patterns of <I>pre et post</I> -treatment with each medicament showed qualitative recovery to that of the normal.