Influence of Polychlorinated Biphenyls on the Lipid Metabolism of Rat:Lipid of Skin and Adipose Tissue
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In our previous investigations, it was elucidated that oral administration of polychlorinated biphenyls (PCB) brought about triglyceridemia in human beings and atrophy of adipose tissue in rats. The purpose of the present study was to investigate, by using rats as an experimental animal, the disturbance of fat metabolism, caused by a long time PCB administration of a small dose.<BR>The rats were fed on 1 mg of PCB daily for 30 days and 1-<SUP>14</SUP>C acetate of 20 μCi was intraperitoneally to the animals thus treated. The rats were killed by decapitation. The back skin and perirenal adipose tissue were cut off. After having been extracted with chloroform-methanol solution from these tissues, the lipid was separated by TLC into phospholipid, free sterol, free fatty acid, triglyceride, and sterol ester. Analysis of lipid by GLC followed. The results obtained were as follows:<BR>(1) Oral administraion of PCB to rats resulted in reduction of free fatty acid and triglyceride in the skin lipid, but it exerted no influence upon the content of phospholipid and total sterol in the rat skin.<BR>(2) Oral administration of PCB to rats revealed a reduction of incorporation of [1-<SUP>14</SUP>C] of acetatate into triglyceride and free fatty acid of the skin lipid, but it promoted the incorporation of <SUP>14</SUP>C) into total sterol.<BR>(3) Nonsaponifiable matters of skin, analyzed and detected by TLC and GLC, were squalene, cholesterol, Δ<SUP>7</SUP>-cholestenol, desmosterol, dihydrolanosterol, and lanosterol. The amount of cholesterol was the highest, and dihydrolanosterol and lanosterol were so little as unable to determine the amounts.<BR>(4) Incorporation of <SUP>14</SUP>C-labeled acetate into squalene was the highest among the nonsaponifiable matters of the rat skin, followed by Δ<SUP>7</SUP>-cholestenol and desmosterol. The incorporation into cholesterol was the lowest.<BR>(5) Upon oral administration of PCB, incorporation of [<SUP>14</SUP>C] into squalene was reduced to one third of the control group. When incorporation of [<SUP>14</SUP>C] into Δ<SUP>7</SUP>-cholestenol and desmosterol was compared with that of the control group, more [<SUP>14</SUP>C] was incorporated into Δ<SUP>7</SUP>-cholestenol than into desmosterol, but this relation was reversed upon administration of PCB. The incorporation of [<SUP>14</SUP>C] into cholesterol was the same both in PCB and control groups.<BR>(6) Incorporation of [<SUP>14</SUP>C] into cholesterol ester fatty acid of rat skin was enhanced by administration of PCB, but other fatty acids in the skin lipid was not influenced<BR>(7) Triglyceride content of the perirenal adipose tissue was reduced markedly upon administration of PCB and when [1-<SUP>14</SUP>C] acetate was injected intraperitoneally, radioactivity was found only in palmitic acid, whereas it was distributed further in longer and desaturated fatty acids in the control group.
- Japan Society of Clinical Chemistryの論文
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