A Microfluorometric Method of Peptides, Amino Acids and Primary Amines by Fluorescent Ninhydrin Reaction

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概要

• 論文の詳細を見る

• A procedure for assay of amino acids, peptides, and other primary amine containing compounds has been developed on the basis of their interaction with ninhydrin and phenylacetaldehyde to yield highly fluorescent ternary products. The fluorescent ninhydrin procedure is 10 to 100 times more sensitive than the colorimetric ninhydrin procedure. The fluorometric reaction has been successfully automated for column chromatography of amino acids and peptides and has been used for detecting primary amine containing compounds on paper and thin-layer chromatograms.<BR>An application has been made for measuring spermidine and spermine contents of rat brain.

• Japan Society of Clinical Chemistryの論文

著者

• 鮫島 啓二郎

  東京大学薬学部

• UDENFRIEND Sidney

  ロッシュ分子生物研究所, U.S.A.

関連論文

• p-アミノアゾベンゼン誘導体の薄層クロマトグラフィー
• A Microfluorometric Method of Peptides, Amino Acids and Primary Amines by Fluorescent Ninhydrin Reaction
• Quantitative Determination of Chinoform in Specimens from Patients with SMON

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