Separation of Albumin into Several Bands in Polyacrylamide Gel Electrophoresis and Abnormal Pattern in Diabetic Serum

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When sera from several mammals put on electrophoresis in polyacrylamide gel containing urea and EDTA, albumin fractions were separated into several bands. Use of purified bovine serum albumin confirmed that the fastest migrating band (Band 1) represented fatty acids-bound albumin and the secondary migrating albumin band (Band 2) coresponds to fat-free albumin. The natures of other bands have remained- obscure so far.<BR>In case of mouse sera, Band 1 appeared only in the fasting phase and disappe- ared at the feeding phase. Bands 3-5 which had not been observed in the fasting phase appeared in 14 minutes after the onset of food uptake and disappeared in 2 hours after food intake. Bands 6-7 appeared only in the last stage of food intake, i. e., in 2-3 hours after meal. Sera from hereditary obese hyperglycemic mice (C57 BL/6J-ob and C57 BL/Ks J-db), however, showed abnormal patterns of serum albumin sub-band, e. g., Bands 3-7 were observed instead of Band 1 in the fasting phase of the ob mice, and Bands 3-5 were not observed even in the feeding phase in the db mice.<BR>Human sera collected at different times after morning meal from a normal male showed a pattern similar to that of the normal mice with regard to albumin sub- band appearance. But the satisfaction (feeding) phase in which Bands 3-5 had appeared continued for more than 11 hours whereas it appeared only for 1 hour in normal mouse sera.<BR>Human sera collected in 60 minutes after oral administration of 50g glucose from diabetic patients failed to show Bands 3-7 which resembled that of the db mice
Japan Society of Clinical Chemistryの論文

Separation of Albumin into Several Bands in Polyacrylamide Gel Electrophoresis and Abnormal Pattern in Diabetic Serum

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