Study on Staining Properties of Acid Mucopolysaccharides on Cellulose Acetate Sheet

概要

論文の詳細を見る
The mode of staining reactions of several AMPS to alcian blue and toluidine blue O was observed on cellulose acetate strips as well as in test tubes.<BR>1. Alcian blue could stain 7 AMPS tested in all buffer conditions except that at pH 7. 0, which indicated its significant validity for qualitative and crude separation procedures.<BR>2. Toluidine blue O stained heparin in reddish violet with a strong metachromasia but chondroitin sulfate A, B and C in blue-violet color with a slight metachromatic property, and heparitin monosulfate as well as keratan sulfate in blue color without any metachromasia.<BR>Hyaluronic acid, however, resulted in differnt reactions by different commercial batches of toluidine blue O, that is, one batch did not react with this material, whereas another did positively.
Japan Society of Clinical Chemistryの論文