A New Method for Determination of Polyamines and Its Application

概要

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A new method for quantitative determination of polyamines in tissues is developed in comparison with the alkaline butanol method which has been widely employed for extraction of polyamines from tissues.<BR>The procedures consist of isolation of polyamines with a small column of Dowex-50 and their separation on paper electrophoresis followed by colorimetric determination after ninhydrin staining.<BR>Recovery by the present method is constantly higher than 90%, with an average value of 95%, whereas the butanol extraction results in variable recoveries ranging from 70 to 90%. Our method is also superior to the butanol method from the standpoint of purity of the isolated polyamines and simplicity of the procedures.<BR>The column method was applied to quantitative estimation of polyamines in various tissues of rat.
Japan Society of Clinical Chemistryの論文