Changes of plasma oxypurine concentrations of whole blood stored at 4°C or 37°C

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The measurement of oxypurines concentrations steadily becomes important because of the usefulness for the diagnosis of hypoxia, ischemic heart disease and malignant diseases as well as the abnormality of purine metabolism like xanthine oxidase defici ency. But question arises as to whether measurement of the oxypurines concentrations in plasma and serum showed the real concentration in the blood, because it has been reported previously that oxypurines concentrations increased in plasma or serum samples when whole blood samples were stored at room temperature for some time without separation in serum or plasma and blood corpuscles. Therefore, we examined changes, in plasma concentration of oxypurines as a function of time for 48 hours between venous puncture and centrifugations of whole blood staying at 4°C or incubatimgat 37°C. We also studied on the cause of difference in plasma oxypurines concentrations between these two groups of samples. Hypoxanthine concentration in plasma was increased even one hour after sampling when whole blood samples were stored at 4°C or at 37°C. In the two groups, however, no significant increase in the plasma concentration of xanthine occurred until 6 hours after venous puncture. Thereafter, both hypoxanthine and xanthine concentrations in plasma continued to increase during the 48-hour period in both, groups. After 48 hours, both hypoxanthine and xanthine concentrations in the plasma were higher in the samples incubated at 37°C than in those stored at 4°C. The increase in the concentrations of these oxypurines was thought to result from purines catabolism in blood cells, catabolism being more accelerated in the samples incubated at 37°C than in those stored at 4°C because the hypoxanthine concentration in blood cells continued to increase along with increase in hypoxanthine concentration in plasma during 48 hours observation, and was always 2 to 5 times as high as that in plasma in both groups. In addition, we investigated whether uric acid, hypoxanthine and xanthine concentrations in plasma increased when platelet rich plasma was stored at 4°C for 24hours, because platelets were known to have xanthine oxidase. After 24 hours, the hypoxanthine concentration in plasma increased significantly but those of uric acid and xanthine did not.
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Changes of plasma oxypurine concentrations of whole blood stored at 4°C or 37°C

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