Stimulation of CCL2 Expression in Human Gingival Epithelium by Candida albicans

スポンサーリンク

概要

• 論文の詳細を見る

• The presence of yeasts in periodontal pockets is well known and Candida albicans (C. albicans) is thespecies most commonly isolated from theoral cavity. Theimmuneresponse and anti-candidal activity of oral epithelium cells play a key role in the host defense against C. albicans infection. Rat gingival tissue was infected with C. albicans ATCC90029. Total RNA was extracted from the epithelium and mRNA levels were monitored using DNA microarray. By ingenuity pathway analysis demonstrated that TNF and IL-1 stimulated gene expression of CC type chemokine CCL2, also known as monocyte chemoattractant protein-1 (MCP-1), through NF-κB pathway. Altered mRNA level of CCL2 by rat gingival tissue was infected with C. albicans was confirmed by reverse transcription polymerase chain reaction. The immunohistochemical examination for CCL2 production was carried out. As a result, stronger immunoreactivity against CCL2 was observed in the rat gingival epithelium infected with C. albicans.

著者

• Abiko Yoshimitsu

  Biochemistry and Molecular Biology

• Nomoto Takato

  Dentistry for the Disabled, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587 Japan

• Kuboyama Noboru

  Oral Molecular Pharmacology

• Hayakawa Eriko

  Biochemistry and Molecular Biology

• Yaguchi Manabu

  Dentistry for the Disabled, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587 Japan

関連論文

• IL-1β production by peptidoglycan from Lactobacillus casei in human dental pulp cells of deciduous teeth and permanent teeth
• Contribution of Porphyromonas gingivalis 40-kDa Outer Membrane Protein to Coaggregation of P. gingivalis Vesicles with Streptococcus gordonji Cells
• Reduction of Matrix Metalloproteinase-3 Expression in Rheumatoid Arthritis Rat Joints by Light-emitting Diode
• Stimulation of CCL2 Expression in Human Gingival Epithelium by Candida albicans

スポンサーリンク