Anticytotoxic Effect of Green Tea Catechin on Aggregatibacter actinomycetemcomitans Vesicles

概要

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One of the potential virulence factors produced by Aggregatibacter actinomycetemcomitans is a leukotoxin, which is secreted to membranous vesicles and to the culture supernatant, generating membrane pores and killing several lymphoid cell types. The lymphoid cells, THP-1 cells exposed to vesicles were lyzed. However, various catechins inhibited the cell lysis by the vesicles. Next, to determine how catchins inhibit vesicles interact with THP-1 cells, SP-DiOC18 labeled vesicles were incubated with catechins and visualized by fluorescens microscopy. Fluorescens microscopy showed that the vesicle did not bind to THP-1 cells in the presence of catechins. These results suggest that the inhibitory mechanisms of catechin on cytotoxic activity could result in the inhibition of binding vesicles to cells by catechins because the catechin component binds to the vesicles.