Development of a Simple, Sensitive and Rapid Quantitative Method for Polycyclic Aromatic Hydrocarbons in Environmental Samples by Thin-layer Chromatography and a Digital Camera

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A simple, sensitive and rapid quantitative method for polycyclic aromatic hydrocarbons (PAHs) in sediment samples was developed by using thin-layer chromatography (TLC) and a digital camera (DC). This method (TLC-DC method) has three-parts: 1. Separation of different types of PAHs on a TLC plate impregnated with a 6 % (w/v) caffeine solution; 2. Excitation of each PAH on the TLC plate by irradiation of a UV lamp and recording of the fluorescence image from each PAH by DC; 3. Analysis of the image data and making calibration curves of PAHs on a personal computer. Anthracene (Anth), fluoranthene (Flu), pyrene (Pyr), benzo[b]fluoranthene ([b]flu), benzo[a]pyrene ([a]pyr), and perylene (Pery) were selected as target compounds. The calibration curves of each PAH obtained in the concentration of 0.01 to 0.3 ppm showed high linearity (R = 0.977 – 0.999). The detection limits for four PAHs (Flu, [b]flu, [a]pyr and Pery) were from 6.8 – 8.8 ng mL−1 (61 – 88 pg per spot), which were almost at the same levels as those by the GC-MS method. Moreover, sediment samples collected at Yoro tidal flat were pretreated. Their PAH concentrations were measured by TLC-DC and GC-MS methods. The PAH concentrations of the sediment samples were 2 – 8 μg kg-dry−1 by the standard addition TLC-DC method, and the values obtained were in good agreement with the values obtained by the internal standard GC-MS method. These results concluded that the TLC-DC method is useful for the quantification of four kinds of PAHs, benzo[a]pyrene, fluoranthene, benzo[b]fluoranthene, and perylene in sediment samples.

Development of a Simple, Sensitive and Rapid Quantitative Method for Polycyclic Aromatic Hydrocarbons in Environmental Samples by Thin-layer Chromatography and a Digital Camera

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