Effect of Maternal Age on the Ratio of Cleavage and Mitochondrial DNA Copy Number in Early Developmental Stage Bovine Embryos

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概要

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• Age-associated deterioration in both the quality and quantity of mitochondria occurs in older women. The main aim of this study was to examine the effect of age on mitochondrial DNA copy number (mtDNA number) in early developmental stage bovine embryos as well as the dynamics of mtDNA number during early embryo development. Real-time PCR was used to determine mtDNA number. In vitro-produced embryos 48 h after insemination derived from Japanese black cows, ranging in age from 25 to 209 months were categorized based on their cleavage status. There was an overall negative relationship between the age of the cow and cleavage status, to the extent that the ratio of embryos cleaved over the 4-cell stage was greater in younger cows. The mtDNA number did not differ among the cleaved status of embryos. In the next experiment, oocytes collected from each donor cow were divided into 2 groups containing 10 oocytes each, in order to compare the mtDNA number of mature oocytes and early developmental stage embryos within individuals. Upon comparing the mtDNA number between oocytes at the M2 stage and early developmental stage 48 h post insemination, mtDNA number was found to decrease in most cows, but was found to increase in some cows. In conclusion, age affects the cleaving ability of oocytes, and very old cows (> 180 months) tend to have lower mtDNA numbers in their oocytes. The change in mtDNA number during early development varied among individual cows, although overall, it showed a tendency to decrease.

著者

• Monji Yasunori

  Tokyo University Of Agriculture

• IWATA Hisataka

  Tokyo University of Agriculture

• KUWAYAMA Takehito

  Tokyo University of Agriculture

• KUWAYAMA Takehito

  Tokyo University of Agriculture, Kanagawa 243-0034, Japan

• MONJI Yasunori

  Tokyo University of Agriculture, Kanagawa 243-0034, Japan

• IWATA Hisataka

  Tokyo University of Agriculture, Kanagawa 243-0034, Japan

• TAKEO Shun

  Tokyo University of Agriculture, Kanagawa 243-0034, Japan

• GOTO Hiroya

  Tokyo University of Agriculture, Kanagawa 243-0034, Japan

関連論文

• Renewal of techniques related to embryo transfer in cattle : a novel superovulation method using aluminum hydroxide gel
• Effect of N-Acetyl-D-Glucosamine on Bovine Sperm-Oocyte Interactions
• Effect of Modification of Ovary Preservation Solution by Adding Glucose on the Maturation and Development of Pig Oocytes after Prolonged Storage
• Effect of Maternal Age on the Ratio of Cleavage and Mitochondrial DNA Copy Number in Early Developmental Stage Bovine Embryos
• Resveratrol Improves the Mitochondrial Function and Fertilization Outcome of Bovine Oocytes

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