The Effects of Adjuvants on Autoimmune Responses Against Testicular Antigens in Mice
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概要
- 論文の詳細を見る
Experimental autoimmune orchitis (EAO) is a model of immunologic male infertility and pathologically characterized by lymphocytic inflammation, which causes breakdown of the testicular immune privilege with spermatogenic disturbance. Generally, murine EAO is induced by immunization with testicular homogenate (TH) from the testes of donor mice + complete Freund's adjuvant (CFA) + Bordetella pertussigens (BP), and it has been considered that treatment with these two adjuvants is required to enhance the immune response against testicular antigens. However, there remains a possibility that CFA and BP may affect autoimmune responses against the testicular antigens without TH. In the present study, we examined this possibility using real-time RT-PCR, Western blotting and immunohistochemical staining. The results demonstrated that immunization with TH in combination with CFA and BP evoked more severe EAO than that with only TH. Real-time RT-PCR analyses revealed that Fas mRNA expression in TH+CFA+BP-induced EAO was significantly higher than that in TH-induced EAO. Interestingly, IL-6 mRNA expression dramatically increased in TH+CFA+BP-induced EAO; however, no apparent change in IL-6 mRNA expression occurred in TH-induced EAO. It was also noted that treatment with CFA and BP alone augmented autoimmune reactions against some testicular autoantigens. These results indicates that these adjuvants are helpful in evoking severe EAO, and treatment with the adjuvants alone can evoke autoimmune reactions against some testicular autoantigens despite the use of no TH.
著者
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QU Ning
Department of Anatomy, Tokyo Medical University
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Terayama Hayato
Department Of Anatomy Tokyo Medical University
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Hirai Shuichi
Department Of Anatomy Tokyo Medical University
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Itoh Masahiro
Department Of Anatomy Tokyo Medical University
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Naito Munekazu
Department Of Anatomy Tokyo Medical University
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Musha Muhetaerjiang
Department Of Anatomy Tokyo Medical University
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HATAYAMA Naoyuki
Department of Anatomy, Tokyo Medical University, Tokyo 160-8402, Japan
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HATAYAMA Naoyuki
Department of Anatomy, Tokyo Medical University, Tokyo 160-8402, Japan
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NAITO Munekazu
Department of Anatomy, Tokyo Medical University, Tokyo 160-8402, Japan
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MUSHA Muhetaerjiang
Department of Anatomy, Tokyo Medical University, Tokyo 160-8402, Japan
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QU Ning
Department of Anatomy, Tokyo Medical University, Tokyo 160-8402, Japan
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QU Ning
Department of Anatomy, Tokyo Medical University, Tokyo 160-8402, Japan
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- Preface
- The Effects of Adjuvants on Autoimmune Responses Against Testicular Antigens in Mice
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