Development of High Sensitive DNA Sensor by Using Probe PNA with IS-FET Electrode

スポンサーリンク

概要

• 論文の詳細を見る

• We demonstrated an ion-sensitive field-effect transistor (IS-FET) based biosensor to directly monitor the hybridization of deoxyribonucleic acid (DNA) using peptide nucleic acid (PNA) as a probe. A single strand DNA or PNA was immobilized as probes on the IS-FET. To detect the hybridization of DNA, we carried out measurements of IS-FET's drain current hybridized by complemental target DNA. Then, the difference voltage was evaluated to be about only 10 mV by using the probe DNA, furthermore monitored as shifts about 170 mV by attempting the probe PNA. The changes in threshold voltage observed for neutral PNA-immobilized IS-FETs were more than 17 times greater than those for negatively charged DNA-immobilized IS-FETs. This approach demonstrates that the PNA-modified IS-FET-based biosensor works more effectively as a signal transducer of genetic information.

• 公益社団法人 電気化学会の論文

著者

• OHTAKE Toshihito

  Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (J

• OHTAKE Toshihito

  Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)

関連論文

• Immobilization of Probe DNA on Ta_2O_5 Thin Film and Detection of Hybridized Helix DNA using IS-FET
• Development of High Sensitive DNA Sensor by Using Probe PNA with IS-FET Electrode

スポンサーリンク