Detection of Direct Electron Transfer between a Redox Enzyme and an Extended Gold-Gate Using Chemical CCD

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概要

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• PQQ-dependent fructose dehydrogenase (PQQ-FDH), which is able to transfer electrons to metal electrodes, was immobilized onto an extended gold-gate of a chemical charge-coupled device (chemical CCD) to measure the catalytic electron transfer in the presence of the substrate. Rapid decrease of the output voltage of the FDH-immobilized gold-gate chemical CCD was observed by the addition of fructose and the extent of the decrease depended on the concentration of fructose. Furthermore, the output profile showed substrate-specificity of FDH. These results strongly suggest that enzymatic oxidation of the substrate and the subsequent direct electron transfer from the enzyme to the gold-gate was detected sensitively with the gold-gate chemical CCD. This finding will open a new field of bioelectronic sensors and devices.

著者

• Shinohara Hiroaki

  Field Of Life Information And System Sciences Graduate School Of Science And Engineering University

• FUJII Akira

  Division of Cardiology, Department of Integrated Medicine and Informatics, Ehime University Graduate School of Medicine

• SISIDO Masahiko

  Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University

• KATO Hirotaka

  Field of Life, Information and System Sciences, Graduate School of Science and Engineering for Research, University of Toyama

• SHINOHARA Hiroaki

  Field of Life, Information and System Sciences, Graduate School of Science and Engineering for Research, University of Toyama

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• Detection of Direct Electron Transfer between a Redox Enzyme and an Extended Gold-Gate Using Chemical CCD

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