Rapid Detection of BSA Protein by Electrochemiluminescence Sensor Combining an Immunoliposome Which Encapsulates a Ru Complex
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概要
- 論文の詳細を見る
An immunoliposome encapsulating a Ru complex with two aminobutyl moieties was prepared to rapidly detect bovine serum albumin (BSA) protein. The detection was carried out within 80 min by electrochemiluminescence (ECL) from the Ru complex adsorbed on Au electrodes after immunoreaction. The adsorption of the complex is a key-step of this procedure in order to increase its sensitivity. Under optimal conditions, the present method can detect BSA in the concentration range from about 1×10−9 g/mL to 1×10−6 g/mL, with almost the same sensitivity as that obtained by conventional ELISA.
著者
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Hifumi Emi
Research Center for Applied Medical Engineering, Oita University
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Hifumi Emi
Research Center For Applied Medical Engineering Oita University:presto Japan Science And Technology
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EGASHIRA Naoyoshi
Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima
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UDA Taizo
Japan Science and Technology Agency, CREST
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HIRATA Takashi
Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima
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HIRATA Takashi
Faculty of Agriculture, Kyoto University
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OHTA Tami
WDB Company
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