Preparation of l-lactate Biosensor that uses Polyion Complex Membrane Containing Peroxidase and Ferrocene

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In order to detect lactate, high sensitivity lactate sensor was completed by attaching lactate oxidase (LOD) to the hydrogen peroxide sensor. The hydrogen peroxide sensor was prepared as follows; poly(4-styrenesulfonate), peroxidase (POD), ferrocene and poly-l-lysine solutions were dropped on a glassy carbon electrode. After drying this electrode, LOD and glutaraldehyde solutions were dropped on the membrane to immobilize the enzyme. The electrode was immersed into a buffer solution (pH 6.5), and a potential of −0.2 V vs. Ag/AgCl was applied to detect the reduction current. Immediately after the addition of l-lactate (10 µM) to the test solution, the reductive current increased and reached a plateau within 20 s. The lower detection limit was 1 µM (S/N=3), and the ratio of the response for the interferent to that for l-lactate on the electrode was 0.18.
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