Sequencing-based Virus Hunting and Virus Detection

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概要

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• Next-generation sequencers have accelerated the advancement of virus hunting or virus detection by metagenomic analysis. Conversely, earlier works enabled virus detection with classic Sanger sequencing by elaborating sample preparation/processing techniques. In this review, we introduce virus hunting technologies both with and without cutting-edge sequencing technology and subsequently discuss the possibility that combining these technologies may extend the application of sequencing-based virus detection from scientific research to everyday diagnostics. Finally, we offer an outlook for another type of technology that leads to pathogen identification using the sequence data obtained in sequencing-based virus hunting.

著者

• Yamaoka Naoto

  Faculty Of Agriculture Ehime University

• SEKINE Ken-Taro

  Iwate Biotechnology Research Center

• KOBAYASHI Kappei

  Faculty of Agriculture, Ehime University

• ATSUMI Go

  Iwate Biotechnology Research Center

関連論文

• Spreading of RNA silencing and DNA methylation in transgenic hybrid plants with the coat protein gene of Sweet potato feathery mottle virus
• Analysis of rice RNA-dependent RNA polymerase 1 (OsRDR1) in virus-mediated RNA silencing after particle bombardment
• Sequencing-based Virus Hunting and Virus Detection
• Gentian Kobu-sho-associated virus : a tentative, novel double-stranded RNA virus that is relevant to gentian Kobu-sho syndrome

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