Deletion Mutations of the Self-incompatibility (S) Locus Induced by Gamma Irradiation in a Wild Diploid Species of Sweet Potato, Ipomoea trifida

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概要

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• Gamma irradiation was used to induce mutations affecting self-incompatibility (SI) in diploid <I>Ipomoea trifida</I>. Plants (<I>S</I><SUB>1 </SUB>homozygote) with developing flower buds were exposed with gamma rays of 10 and 20 Gy. Mature pollen from the irradiated plants was used for crosses with <I>S</I><SUB>3 </SUB>and <I>S</I><SUB>10 </SUB>homozygotes. By pollination test and Southern blot analysis of the M<SUB>1</SUB> progenies, we screened 2 mutant plants carrying a deletion of the <I>S </I>locus derived from irradiated pollen, designated Δ<I>S</I><SUB>1</SUB>. In both mutant alleles, the deleted region was found to exceed a genetic distance of 12 cM around the <I>S </I>locus. Progeny test of both mutant plants showed that neither female nor male gametes carrying the Δ<I>S</I><SUB>1</SUB> allele were fertile probably due to the lethal effect of the chromosomal deletion spanning the <I>S</I> locus.

著者

• Kowyama Yasuo

  Graduate School Of Bioresources Mie University

• Kakeda Katsuyuki

  Graduate School Of Bioresources Mie Univ.

• TSUTSUMI Makiko

  Graduate School of Bioresources, Mie University

関連論文

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• Molecular phylogeny of the genus Hordeum using thioredoxin-like gene sequences
• Expression and functional analysis of the barley Nud gene using transgenic rice
• Deletion Mutations of the Self-incompatibility (S) Locus Induced by Gamma Irradiation in a Wild Diploid Species of Sweet Potato, Ipomoea trifida

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