Establishment of Human Lysozyme Mass Production System Using Insect Factory, Silkworm Larvae

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概要

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• To reduce the manufacturing cost of human lysozyme (HLY) and thus spread its use in medicine, we designed, synthesized, and expressed the <I>HLY</I> gene in silkworm larvae by using a baculovirus system. The production of HLY in silkworms was highly efficient. N-terminal sequencing and mass spectrometry showed that it was physicochemically identical to natural HLY. The lytic activity of recombinant HLY in the silkworm hemolymph was 115 and 154 times that in yeast and silkworm cell culture systems.

• 独立行政法人　国際農林水産業研究センターの論文

著者

• Inumaru Shigeki

  Research Team For Advanced Biologicals National Institute Of Animal Health

• SHIRAI Junsuke

  Department of Exotic Diseases, National Institute of Animal Health, 6-20-1, Josuihoncho, Kodaira, Tokyo 187-0022, Japan

• TSUCHIYA Yoshinori

  Research Team for Advanced Biologicals, National Institute of Animal Health, National Agricultural and Food Organization (NARO)

• INUMARU Shigeki

  Research Team for Advanced Biologicals, National Institute of Animal Health, NARO

関連論文

• Rapid and accurate method for isolation of recombinant baculovirus with an expanded host range(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Effects of Chlorine, Iodine, and Quaternary Ammonium Compound Disinfectants on Several Exotic Disease Viruses.
• Establishment of Human Lysozyme Mass Production System Using Insect Factory, Silkworm Larvae

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