アプタマーサンドイッチ法を用いたVEGFの電気化学的検出

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概要

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• We developed an electrochemical vascular endothelial growth factor (VEGF) detection system using two VEGF-binding aptamers. Sensitive detection systems of VEGF are highly required for cancer diagnosis. In this study, VEGF-A was detected using a sandwiching method with a pyrroquinoline quinone glucose dehydrogenase (PQQ-GDH)-labeled VEGF-binding aptamer and another aptamer immobilized onto a gold wire electrode. We evaluated various combinations of VEGF-binding aptamers to be employed in the process. In addition, we examined dose dependency for the electric current generated by PQQ-GDH, measured in the constructed VEGF detection system. Using this newly constructed system, we successfully detected VEGF165 at 15 nM (M = mol dm−3) concentration.

著者

• ABE Koichi

  Graduate school of engineering, Tokyo University of Agriculture and Technology

• NONAKA Yoshihiko

  Graduate school of engineering, Tokyo University of Agriculture and Technology

• IKEBUKURO Kazunori

  Graduate school of engineering, Tokyo University of Agriculture and Technology

関連論文

• アプタマーサンドイッチ法を用いたVEGFの電気化学的検出

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