An Oxygen Responsive Microparticle-Patterned Hydrogel Sheet for Enzyme Activity Imaging
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概要
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A patch-type oxygen imaging sheet useful for in vitro cellular metabolic assays was developed. Oxygen-responsive fluorescent microbeads were embedded into a biocompatible polyacrylamide gel sheet, which can be directly attached onto target cells for fluorescent imaging of metabolic activity. The sensor beads were directed in a microfluidic device using AC and DC electric manipulation techniques, followed by encapsulation in a hydrogel. Fluorescent imaging of oxygen-consuming activity was demonstrated for glucose oxidase-modified microparticles as cellular models to show the applicability of the imaging sheet to bioassays.
著者
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Nishizawa Matsuhiko
Department Of Applied Chemistry Graduate School Of Engineering Tohoku University
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NAGAMINE Kuniaki
Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University
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TAKEDA Mai
Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University
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ITO Shuntaro
Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University
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OTANI Shingo
Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University
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