Effects of Green LED Lighting on Organogenesis and Superoxide Dismutase (SOD) Activities in Protocorm-like Bodies (PLBs) of Cymbidium Cultured in vitro

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Cymbidium protocorm-like bodies (PLBs) were cultured in modified MS (1962) medium (Shimasaki and Uemoto, 1990) to investigate the effect of light quality on organogenesis in PLBs, and the changes in superoxide dismutase (SOD). Of the different light treatments used in the research, six times of weekly light breaking by one day of green LED lighting (green LED interval lighting) during red LED illumination showed optimal number (14.1) and formation rate (93.1%) of PLB cultures. Optimal shoot formation of PLB cultures obtained at treatments of fluorescent lamp+interval lighting of green LED and blue LED+interval lighting of green LED. The highest fresh weight was achieved under fluorescent lamp condition. Except for red LED lighting treatment, interval lighting of green LED enhanced increase in antioxidant enzyme SOD activity of PLB cultures, and showed the highest SOD activity at blue LED+green LED interval lighting treatment. The study concludes that except for red LED lighting condition, interval lighting using green LED could stimulate proliferation, shoot formation in PLB cultures which accompanying with increase in SOD enzymes.
日本生物環境工学会の論文