Whole Ovary Cryopreservation Applying Supercooling under Magnetic Field

概要

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Chemotherapy and radiotherapy are performed for the treatment of cancer inchildren, especially leukemia. But, the side effects of these tr eatments are a problemof continuing concern, and improving the patients quality of life (QOL) followingsuccessful treatment remains a challenge. Especially with radiation therapy, disordersof reproductive function are considerable, and cases of inferti lity are extremely high.Currently, research on the cryopreservation of ovarian tissue i s being energeticallypursued, but because of factors such as reduction in number of egg cells and physicaldisruption due to tissue fragmentation, the successful fertiliz ation rate of the thawedovum has been extremely low. Accordingly, cryopreservation of t he ovaries as anentire organ has been attempted, but this has yielded little success. This study focusedon a method for cryopreservation through the application of sup ercooling under avariable magnetic field, and the development of a freezing syste m allowing for anarbitrary change in frequency of magnetic field applied to the specimen. To confirm theinterrelation between a variable magnetic field and supercooling, physiological salinesolution was frozen under a variable magnetic field, and the pro gress and stabilizationof supercooling was verified for magnetic field frequencies from 200 to 200 kHz.Additionally, ovaries were frozen under magnetic field, and histological assessment ofthe tissue was performed.