Analysis of Antibody Responses by Commercial Western Blot Assay in Horses with Alveolar Echinococcosis

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概要

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• Commercial western blot (WB) assay was used to detect serum antibodies specific to iEchinococcus multilocularis/i in 23 horses in which infection was confirmed by postmortem inspection at a slaughterhouse. Livers contained from 1 to 20 nodular lesions; foci diameter ranged from 1 to 25 mm. Antibody tests of serum from all 23 animals were negative for antigen bands at 7, 16, 18, and 26-28 kDa, which show specificity in the serum of human patients. However, sera from two infected horses with the largest nodules (diameter, 25 mm) showed positive response to one of the 22-kDa and 30-kDa antigen bands. It may be possible to diagnose iE. multilocularis/i infection in horses based on the detection of these bands on commercial WB assay.

著者

• Kawanaka Masanori

  Department Of Parasitology National Institute Of Health

• UENO Masahiro

  Yamagata Prefectural Nairiku Meat Inspection Center, Yamagata 990-0892, Japan

• KURODA Nobuhiko

  Yamagata Prefectural Nairiku Meat Inspection Center, Yamagata 990-0892, Japan

• YAHAGI Kazue

  Yamagata Prefectural Nairiku Meat Inspection Center, Yamagata 990-0892, Japan

• OHTAKI Toshihiko

  Yamagata Prefectural Nairiku Meat Inspection Center, Yamagata 990-0892, Japan

• KAWANAKA Masanori

  Department of Parasitology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan

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• Analysis of Antibody Responses by Commercial Western Blot Assay in Horses with Alveolar Echinococcosis

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