EXPERIMENTAL STUDIES ON THE UTERINE MUSCULAR ACTOMYOSINATPASE ACTIVITY OF THERA UNDER VARIOUS ENDOCRINOLOGICAL ENVIRONMENTS

概要

論文の詳細を見る
For the purpose of understanding the contractile mechanism of the uterine smooth muscle, actomyosin-ATPase activity of crude extracts of the rat uterus was investigated under various experimental conditions including influences of inorganic ions.<BR>The enzyme activity was more or less altered in the absence of anterior pituitary, adrenal or overy. However, it resumed the level prior to the operation following subsequent treatment with ACTH, desoxycorticosterone and estrogen respectively.<BR>The role of the adrenal seemed to be of interest, especially in the fact that desoxycorticosterone was fairly effective, but not hydrocortisone for recovery of the enzyme activity, if given after the removal of the adrenal. This suggests the importance of electrolytes on the enzymatic activity. From this fact, in bioassay of adrenal contex extract, the survival tes of adrenalectomzied rat might be replaced with the method to determine the actomyosin-ATPase activity of the uterine muscle.<BR>It is conceivable thatcontraction and relaxation of the uterus during the period of gestation and parturition could be more or less explained in relation to the actomyosin-ATPase activity, if the conditions required were thoroughly realzied.<BR>From the results above mentioned at least endocrine glands such as anterior pituitary, adrenal and ovary might be considered to have intimate relation to th enzyme activity concerned.