Repression Method of Algae (<I>Chlorococcales</I>) in Soilless Culture Solution with Ozone
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概要
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The growth of algae (<I>Chlorococcales</I>) in the soilless culture gives a peculiar smell to the products and consumes the nutrient. There is still little desirable method to defend it, so that a method of the algae control by using ozone water, or a solution injected with gaseous ozone, was investigated.<BR>Ozone water, which could restrain the growth of algae, needed higher concentration than that of 0.15-0.4 mg/<I>l </I>which could have effectively inactivated <I>Fusarium</I> fungi from the previous result with petri dish. Namely, the algae mortality of about 90% was obtained at the ozone water concentration of about 1.0 mg/<I>l </I>.<BR>Algae were separated from the solution and were propagated in a test tube under the germfree condition. The ozone water of prescribed concentration was obtained by natural decrease of ozone water of high concentration which was produced from an ozonizer with an oxygen bomb, and had the range of from 0 mg/l to 2 mg/<I>l </I>Algae were suspended with ozone water of various concentrations in the funnel of an aseptic filter of Milipore LTD. for one or three minutes. The treated algae were drew ozone water by a pump and were suspended in sterilized water and then were inoculated on agar.<BR>The practical and effective control methods of algae should be as follows : the solution is treated by injection of ozone when the soilless culture is at rest, or filtered and circulated solution is periodically stored in a tank and exposed to ozone, then returned back to the growth bed after the concentration of ozone water was naturally and fully decreased.