Study on analytical Magnetic Separation system of Bacterial Species

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概要

• 論文の詳細を見る

• Ferrography, a magnetic separation method first developed for industrial sample analyses, has been modefied for application to biological analyses. Separation and quantitation of bacteria in the presence of paramagnetic trivalent metalion, erbium, using modified Ferrography, termed' Bactography', was evaluated to quantitate bacterial concentrations in solutions in the clinical range of interest. Escherichia coli(E.coli)was used as a model bacterial system to optimize the separation protocol. Separation results were evaluated quantitatively by both light scattering and direct cell counting. The modified separation protocol with a newly designed sample processor and magnetic assembly provided higher cell deposition than the previous protocol. The modified protocol and direct cell counting allowed reliable detection and enumeration of concentrations of bacterial suspension of E.coli at105CFU/ml which is of significance in clinical diagnostic applications. Bactography offers promise as a method for quantitation of bacterial solutions.

• ライフサポート学会の論文

著者

• Dohi Takeyoshi

  Department Of Mechano-informatics The University Of Tokyo

• Horiuchi Takashi

  Department Of Chemistry For Materials Faculty Of Engineering Mie University

• DOHI Takeyoshi

  Department of Precision Machinery Engineering, Faculty of Engineering, The University of Tokyo

• TADA Yoko

  Department of Precision Machinery Engineering, Faculty of Engineering, The University of Tokyo

• MALCHESKY Paul

  Department of Biomedical Engineering and Applied Therapeutics, The Cleveland Clinic Foundation, One Clinic Center, 9500 Euclid Avenue, Cleveland, Ohio44195

• ZBOROWSKI Maciej

  Department of Biomedical Engineering and Applied Therapeutics, The Cleveland Clinic Foundation, One Clinic Center, 9500 Euclid Avenue, Cleveland, Ohio44195

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