The determination of alkaline phosphatase activity in serum by means of flow-through analysis with an ion-selective electrode.
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概要
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In order to determine continuously the alkaline phosphatase activity in a serum sample, a potentiometric method using a newly prepared phosphate ion-selective electrode has been established by the authors for the first time. The alkaline phosphatase activity was determined by measuring the concentration of the phosphate ion which was liberated by the enzymatic hydrolysis of disodium phenyl phosphate in the presence of alkaline phosphatase. The measurement of the phosphate ion with an ion-selective electrode was seriously interfered with by protein and the halide ion, but the interference could be removed by covering the electrode surface with a cellulose acetate membrane and by separating the chloride ion from a serum sample by gel chromatography. The present method was applied to the determination of alkaline phosphatase activity in serum; the results obtained showed a satisfactory correlation with those obtained by the conventional method. The method was found to be useful for practical diagnosis based on the activity of alkaline phosphatase.
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