Solid-phase synthesis of substrate model peptides and their hydroxylation with collagen lysyl hydroxylase.

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概要

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• The peptides, (Ala–Lys–Gly)<SUB>10</SUB>–Phe, (Ile–Lys–Gly)<SUB>3</SUB>–Phe, and (Ile–Lys–Gly)<SUB>5</SUB>–Phe, were synthesized by solid-phase fragment condensation method. The contaminating shorter peptides and branched chain peptides were removed by CM-cellulose column chromatography. The peptides were useful as synthetic model substrates for collagen lysyl hydroxylase. The –Ile–Lys–Gly– units in (Ile–Lys–Gly)<SUB>5</SUB>–Phe showed a higher affinity to the enzyme than those in (Ile–Lys–Gly)<SUB>3</SUB>–Phe. A strong substrate inhibition was observed with highly basic peptide, (Ala–Lys–Gly)<SUB>10</SUB>–Phe.

• 公益社団法人 日本化学会の論文

著者

• Kikuchi Yasuo

  Department Of Biomolecular Sciences Graduate School Of Life Science Tohoku University

• Tamiya Nobuo

  Department of Chemistry, Faculty of Science, Tohoku University

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