Studies of polystyrene-based ion-exchange fiber. IV. A novel fiber-form material for adsorption and immobilization of biologically-active proteins.

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概要

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• A new polystyrene-based ion-exchange fiber (IONEX) has a large surface area per unit weight and has been studied for its ability to adsorb and immobilize biologically-active proteins. A strong cation IONEX was found to be able to effectively adsorb hemoglobin and albumin, invertase, and glucose isomerase were readily adsorbed to a strong anion IONEX. The adsorption of these proteins to IONEX was found to take place through an electrostatic force. The adsorption capacity for proteins became exceedingly large (ca. 300 mg g<SUP>−1</SUP>) with an increase in the water-holding capacity of the fiber. The activities of the invertase and glucose isomerase immobilized to the fiber exhibited 40–50 and 75–80%, respectively, of those of native enzymes. A continuous inversion of sucrose was also carried out using immobilized invertase. From these results, this fibrous ion exchanger with a high water-holding capacity is considered to be an excellent material for the adsorption and immobilization of proteins.

• 公益社団法人 日本化学会の論文

著者

• Shimamura Masaharu

  Fibers Research Laboratories, Toray Industries, Inc.

• Yoshioka Toshio

  Fibers Research Laboratories, Toray Industries, Inc.

関連論文

• Studies of polystyrene-based ion exchange fiber. I. The preparation and fundamental characteristics of polystyrene-based ion exchange fiber.
• Studies of polystyrene-based ion-exchange fiber. IV. A novel fiber-form material for adsorption and immobilization of biologically-active proteins.
• Studies of polystyrene-based ion-exchange fiber. V. Immobilization of microorganism cells by adsorption on a novel fiber-form anion exchanger.

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