Nucleotide sequences of DNA and their structural analysis. Categorical discrimination of 5'-splice site sequences in mRNA precursors.
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The signals which direct excision of introns from mRNA precursors in higher eukaryotes' genes are not well-understood. Although a consensus sequence, (<U>Remark: Graphics omitted.</U>), has been proposed with the 5′-splice site, actual 5′-splice site sequences differ from it to a greater or lesser degree. In the present paper, structural analysis of 5′-splice site sequences was done by using quantification theory (class II). Nucleotide sequences were transformed into categorical data, and categorical discriminant analysis was carried out between 5′-splice site sequences and sequences other than 5′-splice site. Relative importance of each position of nucleotide sequence was then estimated by calculating partial correlation coefficient on each position.
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