Study on conjugated redox membranes containing an intercellular component of wool as an electron carrier.

概要

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To realize artificially such a conjugated redox reaction as that which is drived across the biological cell membrane, two enzymic redox reactions were conjugated across a hydrogel membrane prepared with urethane prepolymer holding stabilized isocyanate groups. As redox couples, glucose 6-phosphate (G6P)/glucose 6-phosphate dehydrogenase (G6PD)/gluconolactone 6-phosphate (GL6P) and glutathione oxidized form (GSSG)/glutathione reductase (GRD)/glutathione reduced form (GSH) were chosen together with their common coenzyme, NADP+/NADPH. The driving of a system consisting of two redox couples separated with the hydrogel membrane containing the coenzyme NADP+ was intiated by the reduction of NADP+ on the membrane surface in contact with the G6PD/G6P solution. The conjugated redox reaction proceeded by electron exchange between coenzymes in the membrane and the reproducing of NADP+, which was accompanied with the GRD/GSSG solution. The rate of electron transfer across the urethane membrane was markedly accelerated by incorporating a nonkeratinous intercellular cement compound extracted from wool into the membrane. From cyclic voltammograms of a methyl viologen mediator, using a bare electrode and an intercellular compound modified carbon electrode, it was confirmed that the intercellular component could surely act as a good electron carrier.
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