STUDIES ON MONOAMINE OXIDASE (REPORT XXXIV).EFFECTS OF AMMONIUM SULFATE ON MITOCHONDRIAL MAO IN BEEF LIVER
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The effects of ammonium sulfate on mitochondrial monoamine oxidase (MAO) in beef liver were studied. The initial stage of the reaction was measured with an oxygen electrode and the long term reaction was measured manometrically. With tyramine or benzylamine as substrate, addition of ammonium sulfate increased the initial reaction but decreased the long term reaction. Ammonium sulfate had similar effects on solubilized MAO. Inhibition of MAO activity in both the initial and long term reaction by excess substrate was diminished by addition of ammonium sulfate. The effects of ammonium sulfate on MAO were reversible. Salts having an -SO<SUB>4</SUB> group, such as Na<SUB>2</SUB>SO<SUB>4</SUB> and K<SUB>2</SUB>SO<SUB>4</SUB>, increased MAO activity in the initial reaction while salts having an -NH<SUB>4</SUB> group, such as NH<SUB>4</SUB>Cl and NH<SUB>4</SUB>NO<SUB>3</SUB>, inhibited it. However, all these salts inhibited MAO activity in the long term reaction. The mechanism of activation of MAO activity by addition of ammonium sulfate is discussed.
- 公益社団法人 日本薬理学会の論文