RadioimmunoassayによるAndrostenedioneの測定:第1報 Testosteroneとの同時測定法について

概要

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Radioimmunoassay using the antiserum of Δ<SUP>4</SUP>-Androstenedione-3-oxime-BSA for estimaton of androstenedione is described. Assay of plasma volume is an adequate 0.2 ml in the adult male. <BR>Radiochemical isolation of androstenedione and testosterone simultaneously was achieved with Sephadex LH-20 microcolumn chromatography. <BR>With this method, water blank controls showed values of 7.7 ± 4.0 pg/sample (N=5) in androstenedione and 93.3 ± 9.4 pg/sample (N=5) in testosterone. Normal adult male plasma contain 858.8 ± 225.7 (N=5) pg/ml (N=5) in androstenedione and 6.94±1.09 ng/ml (N=5) in testosterone.
日本内分泌学会の論文