ヒト副腎遊離細胞におけるステロイド産生に果すカルシウムの役割
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概要
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The present experiment was planned to verify the effect of calcium on adenyl cyclase in isolated human adrenal cells.<BR>Normal adrenal glands were obtained surgically from patients with primary aldosteronism and advanced breast cancer. Isolated adrenal cells were prepared by the modified Haning's method. They were incubated at 37°C under a gas mixture of 95% O<SUB>2</SUB> : 5% CO<SUB>2</SUB> in calcium-free Krebs-Ringer bicarbonate buffer solution containing 0.2% glucose and 0.5% fatty acid-free bovine serum albumin, to which various doses of CaCl<SUB>2</SUB> or ACTH were added.<BR>Thirty minutes later, cyclic-AMP was measured by cyclic-AMP assay kit (The Radiochemical Center, Amersham). 11-OHCS was estimated fluorometrically by the modified Silber's method after incubation for 2 hours<BR>In the calcium-free incubation medium, productions of 11-OHCS and cyclic-AMP were negligible. In the concentration of 2.54 mM/L of calcium, 11-OHCS production increased with significant difference statistically, while the increase of cyclic-AMP production was not significant. In the concentration of 12.70 mM/L of calcium, however, cyclic-AMP production increased remarkably. When ACTH was added to the incubation medium containing 2.54 mM/L of calcium, productions of 11-OHCS and cyclic-AMP also increased remarkably.<BR>These results indicate that adenyl cyclase of human adrenocortical cells is directly stimulated by calcium and sggest that calcium acts as the second messenger of ACTH.
- 日本内分泌学会の論文