Estronesulfateの生体内動態とその意義

概要

論文の詳細を見る
It is well known that estrone sulfate (E1-S) is a major estrogen in human serum; however, the physiological role of E1-S is not yet clarified. In order to elucidate this physiological role, the following experiments were performed. 1) The conjugation and metabolism of E1-S in the Japanese monkey (Macaca fuscata) Following an injection of the equimolar mixture of [4-14C] E1 and [6, 7-3 H] E1 -S into a femoral vein of the Japanese monkeys, urine, blood (from a femoral artery) and bile were collected at various time intervals over a period of 2 hs. Various tissues, that is, kidney, liver, lung, endometrium, fatty tissue, myometrium and skin, were taken after sacrifice. The metabolites were analyzed by DEAE Sephadex A-25 column chromatography, enzyme hydrolysis and TLC. E1, E1-glucosiduronate (E1-G) and E1-S were identified in the serum, and E1, E1-G, estradiol-17α-3G (E2-17α-3G), estradiol-17β-3G (E2 -3G), E1 -S and E2-17α-S were identified as urinary metabolites. In the bile, 16α-hydroxy-estrone-G was also present. A large amount of [3H] E1 -S was present in the early collection of the serum, and [3H] E1-G gradually increased later. 14C, which was present less than 3 H in the serum was conjugated to [14 C] E1-G and [14 C]E1 -S later. Namely, E1 -S was one of the conjugated forms of E1 in the serum. There was much 14C and less 3H in the lung tissue. Therefore, one of the physiological roles of E1-S is to pass through the pulmonary circulation as compared to E1. E1 -S and E1 were conjugated into E1 -G and E1 -S in the general circulation. Glucosiduronate of estrogens was mainly excreted into the urine. 2) Renal conjugation and metabolism of E1 and E1-S Following the injection of labelled estrogen into one of the renal arteries, urine was collected from both kidneys. Urinary metabolites were analyzed using the same methods as above.1 Injection of [4-14C] E1 into one of the renal arteries and [6, 7-3H] E1 into a peripheral vein. A larger amount of [14C] E1 -G was identified in the injected side urine in the early period (5-10 min) rather than [3 H] E1 -G. This denotes the formation of glucosiduronation in the kidney of the Japanese monkey in vivo. 2 When [4-14C] E1 and [6, 7-3 F-3] E1 -S were injected into one of the renal arteries of the monkey, El-S was partially filtered from the kidney directly, but no excretion of E1 into the urine was detectable. It was shown that E1-S was hydrolyzed and then was conjugated into E1 -G in the general circulation, and after a while the E1 -G was filtered from the kidney. 3 Following the injection of [4-14C] E1 and [6, 73H] E1-G into one of the renal arteries, E1-G was filtered very quickly from the kidney even compared to E1-S. 3) Hydrolysis of E1-S in the endometrium The mixture of [6, 73 FI] E1 -S and [4-14C] E1 was incubated with human endometrium. The ethanol extract was analyzed by DEAE Sephadex A-25 column chromatography, enzyme hydrolysis and TLC. Myometrium, fatty tissue and muscle were also incubated as the control. The results were as follows : The hydrolysis of E1 -S in the endometrium, target organ of estrogen, was 19.3% and those in the fatty tissue, muscle, and myometrium were 5.8, 3.3, 1.9% respectively.
日本内分泌学会の論文

Estronesulfateの生体内動態とその意義

スポンサーリンク

概要

著者

関連論文

スポンサーリンク