成長異常小児における血清GHのradioimmunoassayとradioreceptorassayによる検討
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概要
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A radioreceptor assay (RRA) for human growth hormone, using a 100,000g pellet from late pregnant rabbit liver homogenates, was reported by Tsushima, T. and Friesen, H.G. and applied clinically to the study of serum GH in patients with acromegaly. We studied the serum GH of children with growth disorders with RRA and RIA. The GH receptor used for the RRA was prepared according to the method of Tsushima, T. and Friesen, H.G. Iodination of hGH with <SUP>125</SUP>I is commonly performed using the lactoperoxidase method, but in our study it was prepared by the chloramine T method as reported by Tsushima, T. and other authors. The specific activity of the hGH tracer was 40-60 μKi/μg. The GH RRA was performed by the method reported by Sneid, D.S. et al. with slight modification. Under these conditions, the sensitivity of the assay was 1.8 ng/ml. RRA interassay variation was 4.2% at 22 ng/ml of serum GH (RIA). The cross reactivity of human prolactin and TSH in this system was not detected, but that of LER-907 was 5%. The mean RRA/RIA ratio of the contaminated GH in LER-907 was 1.050 ± 0.15. The purified hGH, prolactin, TSH and LER-907 used in these studies were provided by the National Institutes of Arthritis, Metabolism and Digestive Diseases.<BR>Due to the limitation in RRA sensitivity and the difference between RIA and RRA, the quantity of GH in low levels of serum GH (lower than 2 ng/ml) was not studied. The mean RRA/RIA ratio of 8 sera from 6 normal adults was 1.37 ± 0.30, ranging from 0.9 to 1.8. In 15 patients with constitutional dwarfism and 8 patients with delayed adolescence, these ratios were 1.236 ± 0.95, ranging from 0.53 to 2.2, and 1.284 ± 0.308, ranging from 0.7 to 1.63. In 4 patients with cerebral gigantism, which is a syndrome consisting of a nonprogressive neurological disorder with mental retardation, advanced height from birth, and skeletal maturation, this ratio was 1.335 ± 0.412. There did not seem to be an abnormal GH in the circulation, which had a greater biological potency than immunological one in this disorder. Some differences in our results as compared to those previously reported by Sneid, D.S. et al. could be explained by the use of different iodination methods.
- 日本内分泌学会の論文
著者
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前坂 機江
神奈川県立こども医療センター内分泌代謝科
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諏訪 誠三
神奈川県立こども医療センター小児科
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前坂 機江
神奈川県立こども医療センター 内分泌代謝科
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諏訪 誠三
神奈川県立こども医療センター 内分泌代謝科
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