In vitroに於ける, 合成ovine CRFのACTH放出活性
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概要
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The effect of the synthetic ovine corticotropin-releasing factor (CRF) on adrenocorticotropin (ACTH) release was examined by the perifusion method using rat anterior pituitary tissue and rat monolayer cultured pituitary cells.<BR>Quartered anterior pituitaries were placed in a chamber and perifused at a rate of 400 μl/min with Dulbecco's modified Eagle Medium (DMEM, pH 7.4) bubbled with a mixture of 95% O<SUB>2</SUB> and 5% CO<SUB>2</SUB>. The perifused medium was fractionated, and the ACTH concentration was measured by radioimmunoassay.<BR>In the monolayer cultured pituitary cells, the amount of ACTH released in the culture medium during three hours incubation was assayed by radioimmunoassay.<BR>ACTH was released from the perifused anterior pituitary in a dose-related manner by the pulse administration of CRF or arginine vasopressin (AVP) at the concentration of 1 ng/ml, 10 ng/ml and 100 ng/ml. A significant difference was not found between CRF-and AVP-induced ACTH release.<BR>In the monolayer cultured pituitary cells, synthetic ovine CRF induced ACTH release in a dose-related manner between 30 pg/ml and 30 ng/ml, but AVP induced a slight ACTH release.<BR>ACTH release was pulsatile during the continuous administration of 2.5 ng/ml of CRF for 150 min, although if gradually increased during the continuous administration of 10 ng/ ml or 20 ng/ml of CRF. The continuous administration of AVP also caused pulsatile ACTH release at 10 ng/ml, but the ACTH release gradually decreased during the continuous administration of AVP.<BR>The interaction between CRF and AVP on ACTH release was examined by two methods. When CRF and AVP were given simultaneously, a mainly additive effect on ACTH release was observed. However, a low concentration of CRF seemed to potentiate AVP-induced ACTH release.<BR>These results show that both CRF and AVP have a significant CRF activity on the perifusion system, that AVP induced a slight ACTH release in monolayer cultured pituitary cells, and that CRF acts additively or potently with AVP to control the ACTH release from the anterior pituitary gland.
- 日本内分泌学会の論文
著者
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太田 善介
岡山大学・第3内科
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高原 二郎
岡山大学・医学部第三内科
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村上 和春
岡山大学・医学部第三内科
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橋本 浩三
岡山大学・医学部第三内科
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景山 甚郷
岡山大学・医学部第三内科
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太田 善介
岡山大学・医学部第三内科