Molecular Genotyping of Mycobacterium tuberculosis in Mie Prefecture, Japan, Using Variable Numbers of Tandem Repeats Analysis

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概要

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• The variable numbers of tandem repeats (VNTR) analysis is a method frequently employed as a molecular epidemiological tool for Mycobacterium tuberculosis genetic fingerprinting. In this study, we characterized the population of M. tuberculosis circulating in Mie Prefecture, Japan, and assessed the utility of the proposed JATA12- and 15-VNTR analyses of 158 M. tuberculosis clinical isolates using 25 VNTR loci. The results revealed that the ancient Beijing sublineage is the most prevalent M. tuberculosis strain in Mie Prefecture, accounting for 85.0% of 113 Beijing lineage isolates. Our results also showed that JATA-VNTR using well-selected loci is as reliable as standardized 15-locus MIRU-VNTR. Furthermore, JATA15-VNTR analysis reliably improved the discriminatory power compared with basic JATA12-VNTR analysis. In summary, our data suggest that JATA-VNTR is a useful tool for discrimination of M. tuberculosis in areas where ancient Beijing strains are frequently isolated.

著者

• Nosaka Tetsuya

  Department Of Hematopoietic Factors Institute Of Medical Science University Of Tokyo

• Sakai Takashi

  National Hospital Organization Mie-Chuo Medical Center

• Iwade Yoshito

  Mie Prefecture Health and Environment Research Institute

• Tanuma Masamichi

  Mie Prefecture, Department of Health and Welfare

• Nosaka Tetsuya

  Department of Microbiology and Molecular Genetics, Mie University Graduate School of Medicine

• Nagai Yuhki

  Mie Prefecture Health and Environment Research Institute

• Hayakawa Eri

  National Hospital Organization Mie-Chuo Medical Center

• Nakano Manabu

  National Hospital Organization Mie-Chuo Medical Center

• Katayama Masahiko

  Mie Prefecture Health and Environment Research Institute

• Yamaguchi Tetsuo

  Mie Prefecture Health and Environment Research Institute

関連論文

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• Janus Kinases (JAKs) and Signal Transducers and Activators of Transcription (STATs) in Hematopoietic Cells
• Possible involvement of RasGRP4 in leukemogenesis
• Molecular Genotyping of Mycobacterium tuberculosis in Mie Prefecture, Japan, Using Variable Numbers of Tandem Repeats Analysis

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