2 人胎児におけるTSH
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概要
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I. Plasma TSH in pregnant women.<BR>Plasma TSH concentrations in 38 pregnant women from 8 to 32 weeks' gestation ranged in the levels of normal subjects (≤5.0 μU/ml) determined by HTSH radioimmunoassay.<BR>II. Human Chorionic Thyroid-Stimulating factor.<BR>The partial purification and immunological characteriaztion of the human chorionic thyroid-stimulating factor was described. Bates' percolation method and CM-cellulose chromatography were applied to normal human placenta, and allows the detection of human chorionic thyroid-stimulating factor by Mckenzie's bioassy. Multiple doses of the Bates' fraction and. CM fraction resulted in curves not parallel with that obtained by human TSH standard. The biological activity of human chorionic thyroid-stimulating factor was not neutralized by the in vitro addition of anti-bovine TSH serum. On the other hand, no significant effects were observed when Bates' fraction and CM fraction were assayed by the HTSH radioimmunoassay.<BR>III. TSH in the human fetal pituitaries.<BR>TSH concentration in human fetal pituitaries was determined by radioimmunoassay and the McKenzie's bioassay. In 40 human fetal pituitaries from 8 to 32 weeks' gestation, TSH determined by radioimmunoassay was first detectable at 12 weeks' gestation, and the content increased progerssively with fetal age and reached about 1/10 of TSH content in adult pituitary glands at 32 weeks' gestation. TSH content per fetal body weight was calculated and found to be maximum at 12 to 17 weeks' gestation. On the other hand, TSH activity determined by the McKenzie's bioassay in 30 human fetal pituitaries from 8 to 32 weeks' gestation was first detectable at 14 weeks' gestation. The ratio of bioassay potency (B) /radioimmunoassay potency (I) was 6.65 (mean) (in control experiment, the ratio of B/I of 5 adult pituitaries was 4.5), when Human Thyrotropin Research Standard A was used as a standard in both assays.
- 日本内分泌学会の論文