過ヨウ素酸ナトリウム酸化法による小児の尿中17-Ketogenic Steroids (17KGS) 分画に関する研究特に健康小児の尿中17-KGS, 17-KS, Pregnanetriol排泄量について
スポンサーリンク
概要
- 論文の詳細を見る
1. Preliminary experiments on the determination of urinary 17-KGS fractions.<BR>The procedure employed was Few's method partially modified by Kanbegawa. The urine was treated with sodium borohydride, sodium periodate, then extracted with ethylether and fractionated into 2 parts of 11-deoxy-17-KGS and 11-oxy-17-KGS by means of column chromatography on alumina, and finally determined with Zimmermann's reaction. Satisfactory results were obtained in the separation of both fractions, the recovery ratio and the reproduction with 7 kinds of pure reference C-21-steroids (Fig. 2, Table 1, 2). After studying Zimmermann's reaction, it was found that method B is suitable for the determination of both fractions (Table 3).<BR>2. Determination of the 24-hour urinary excretion of the 17-KGS fractions was carried out in 55 normal children (27 males and 28 females) whose ages ranged from 3 to 15 years and in 10 normal adults. A gradual increase according to the age was observed on both fractions. The value per body surface unit was calculated. An approximately constant value was obtained in the group of children under 12 years old and over 13 years old, respectively. Similar tendencies were observed on the basis of body weight. As the value per body weight ranged more widely than those per body surface unit, the latter is more suitable as a normal standard. No sex differences were noted in the group of children between 3 to 12 years of age while sex differences were noted in the group over 13 years old (Fig. 4, 6, 7, Table 4a). 11-deoxy-17-KGS/11-oxy-17-KGS ratio was 0.22±0.14 (5% rejection limit) with no differences in relation to age and sex (Fig. 5, Table 5).<BR>3. Determination of the 24-hour urinary excretion of the total 17-KS was also carried out in the same subjects. Similar tendency of the gradual increase with age noted in 17-KGS was also observed in 17-KS. However, the values per body suface were approximately constant in the group of children under 12 years old, but not in the group over 13 years old. Age and sex differences were observed in the latter group (Fig. 9, Table 4a).<BR>4. Urinary pregnanetriol was determined in 19 cases of normal children by the method of Bongiovanni-Eberlein and the values were compared with those of 11-deoxy17-KGS. Pregnanetrol/11-deoxy-17-KGS ratio was 0.44 (mean) ±0.23 (standard deviation), which seemed to increase according to the age over 13 years old (Fig. 8. Table 4b).<BR>5. Positive correlation was observed between 17-KGS and 17-KS. However the KS values were relatively larger in the group over 13 years old than in children under 12 years old (Fig. 10).
- 日本内分泌学会の論文