Hydrocortisone, Dexamethasone および Paramethasoneによる副腎萎縮の組織学的および酵素組織化学的研究
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Adrenocortical atrophy of the male Wistar rat, induced by daily injetions of hydrocortisone 10 mg/kg, paramethasone 0.5 mg/kg and dexamethasone 0.25 mg/kg, respectively, for 5 days and 1, 2, 3 and 5 weeks, was studied by histologic, histometric and enzyme histochemical procedures.<BR>Significant reduction in weight of the adrenal gland and in width of the cortex which was, in particular, involved in the diminshing in length of the fascicular and reticular layers, were detected in the dexamethasone groups. These atrophic changes were less evident in the paramethasone groups and very slight in the hydrocortisone groups.<BR>In the dexamethasone groups, the conspicuous atrophy of the fascicular and reticular layers appeared within 5 days to 1 week. Degeneration atrophy in the adrenal cortex was accelerated by the prolongation of the daily injections. In 2 and 3 weeks, necrosis and necrobiosis were particularly profound in the reticular layer, which was followed by zonal fibrosis in 5 weeks. In the paramethasone groups, there were no severe cortical lesions resulting in fibrosis. The atrophic changes continued to be slight in the hydrocortisone groups.<BR>In the dexamethasone groups, the activity of glucose-6-phosphate dehydrogenase was suppressed moderately in the fascicular and reticular layers within 5 days to 1 week, and the change was evident in the animals of 2 to 5 weeks. The loss of glucose-6phosphate dehydrogenase activity was less severe in the paramethasone groups and very slight in the hydrocortisone groups.<BR>In the dexamethasone groups, accumulation of intracellular coarse lipid globules appeared in the fascicular and reticular layers within 5 days to 1 week. In the paramethasone groups, the same change was demonstrable within 1 to 2 weeks, and in the hydrocortisone groups within 5 days to 1 week. Intracellular lipid globules were gradually decreased in size and in density by the prolongation of daily injections. The animals with in 5 weeks of dexamethasone injection had only a small amount of faint lipid droplets within the cortical cells. The decrease of cortical lipids was less severe in the paramethasone groups and very slight in the hydrocortisone groups.<BR>The activity of alkaline phosphatase in the capillary wall in the fascicular and reticular layers was reduced markedly in 1 week in the dexamethasone group and moderately in 2 weeks in the paramethasone and hydrocortisone groups. In these animals, the reduction of the activity was considered to be in reliable relation to the density of coarse lipid globules in the cortical cells. The reduction of the alkaline phosphatase activity was gradually mended by the prolongation of the daily injection in all experimental groups, and in 5 weeks the activity was the same as that in normal control animals. It was suggested that the mending of the reduction of the alkaline phosphatase activity was induced by the release of the intracellular lipid material and of cortical cell debris into the blood stream through the capillary wall.<BR>The activity of triphosphopyridine nucleotide diaphorase was slight but had a similar pattern to that of glucose-6-phosphate dehydrogenase. There were no reliable changes in the activities of acid phosphatase, adenosine triphosphatase, nonspecific esterase, succinic dehydrogenase, and 9 other dehydrogenases and diaphorases in all experimental groups.
- 一般社団法人 日本内分泌学会の論文