Thyrotoxic myopathyの燐酸代謝に関する研究
スポンサーリンク
概要
- 論文の詳細を見る
Phosphorus metabolism and creatine phosphokinase activity in muscle of thyrotoxicosis were studied biochemically. Serum creatine phosphokinase activity and significance of these abnormalities in relation with clinical signs and symtoms in thyrotoxic myopathy were also investigated. In addition, similar investigations were done in experimentally produced tyrotoxic rats for confirmation of the results obtained from clinical materials.<BR>Method : <BR>Inorganic phosphorus (IP), creatine phosphate (CP), adenosine triphosphate, measured as Δ 10 P (ATP) and creatine phosphokinase activities were measured directly in frozen muscle tissu, prepared in saturated dry-ice acetone solution.<BR>Material were obtained from 102 patients with thyrotoxicosis, and as controls 44 patients with simple goiter and 19 normal subjects.<BR>Diagnosis of thyrotoxicosis was established clinically, and BMR, PBI, I<SUP>131</SUP> uptake were carefully studied in the laboratory.<BR>Biopsy specimens were obtained from sternocleidomastoid muscles under local anesthesia with 0.5% novocain solution. Serum was taken during fasting state in the morning and materials were measured biochemically as soon as possible.<BR>Experimental thyrotoxic rats were produced by subcutaneous injection of thyroxine 2 mg/kg for 3 weeks and sacrified for the analysis as described previously.<BR>Results : <BR>(1) In muscles of the thyrotoxic patients and the controls, inorganic phosphorus levels were not different materially. Howerer, creatine phosphate and adenosine triphospate values were lower in muscles taken from patients with hyperthyroidism. The 44 per cent decrease in creatine phosphate and the 26 per cent drop in adenosine triphosphate were both highly significant statistically.<BR>(2) Serum creatine phosphokinase were measured in 61 thyrotoxic patients, 18 none-toxic goiter and 15 normal subjects. These mean values were 5.9±4.6 (u/hr/ml), 3.3±1.7 and 2.4±1.7. Creatine phosphokinase activities in serum in thyrotoxicosis was elevated significicantly.<BR>(3) Muscle creatine phosphokinase activities were measured in 27 thyrotoxic patients, 16 none-toxic goiter and 4 normal subjects. Creatine phosphokinase activities were diminished in one third of the muscles taken from thyrotoxic patients but the difference was not of statistical significance.
- 日本内分泌学会の論文