Wistar系ラツトの両親糖尿病持続期間とその子のLangerhans氏島β細胞発育障害との関係について
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Recently it has been reported by K. Okamoto and his coworkers that disorders in the development of β-cells in Langerhans' islets are observed in the ofispring of Wistar strain rats with long-continued alloxan diabetes (both parents). It has also been reported that abnormalities are seen in the off-spring of normal females and males which have been diabetic for at least 23 days, or of normal males and females which have been diabetic for at least 24 days. <BR>To investigate the duration of diabetes reqired to bring about disorders in the β-cells in offspring of both diabetic parents, the following experiment was performed. <BR>Male and female rats of the Wistar strain with alloxan diabetes of varying durations were mated. <BR>The offspring which weighed over 150 g on the 90th day after birth were sacrificed by ether. Gomori's stain was applied to pancreatic tissues, and the number of, and the area of β-or α-cells in Langerhans' islets were determined. <BR>1) In 30 normal rats (15 males, 15 females) the number of β-cells in one islet was found to be 65.4-70.4 (average 67.7 ± 1.5), the area of β-cell was 108.9-118.3, μ<SUP>2</SUP> (average 113.1 ± 2.8μ<SUP>2</SUP>), the number of α-cells was 14.0-49.6 (average 15.6 ± 1.8) and the area of α-cell was 58.9-67.4μ<SUP>2</SUP> (average 62.6 ±2.3 μ<SUP>2</SUP>). There was no difference between males and females. <BR>2) In all of the offspring of males and females which had both diabetes for 7 days (4 cases), 14 days (6 cases) or 22 days (4 cases), the number and the area of β- or α-cells were normal. <BR>3) In all of the offspring of rats, diabetic for over 40 days, the number of β-cells was marked by decreased (46.6-50.4, average 48.4±1.2), and the area of β-cell was reduced (average 107.2±1.9μ<SUP>2</SUP>). The number of α-cells showed a slight decrease (average 13.8 ± 0.6), and the area of α-cell showed a slight reduction (average 57.2 ± 1.6μ<SUP>2</SUP>). <BR>4) The number of β-cells in 3 of the 7 offspring of rats, diabetic for 23 days, was normal, and in the other 4 it was decreased (57.4-61.6, average 59.9 ± 1.6). The amount of decrease was half that seen in the offspring of rats with diabetes for over 40 days. The area of β-cell showed a slight reduction (average 108.7 ± 1.3μ<SUP>2</SUP>). The number and the area of α-cells were normal. <BR>5) The number of β-cells in the 5 offspring of rats with diabetes for 24 days was decreased, in 2 to an average of 50.5, which is as low as in the offspring of rats with diabetes for over 40 days, and in 3 to an average of 58.4, of which the amount of decrease was half of that seen in the off-spring of rats with diabetes for over 40 days. The area of β-cell was slightly reduced (average 108.3 ± 1.2μ<SUP>2</SUP>). The number of α-cells was normal in some and somewhat decreased in others (average 13.4). The area of α-cell was reduced a little (average 58.6 ± 2.0μ<SUP>2</SUP>). <BR>6) The number of β-cells in all of the offspring of rats with diabetes for 27 days, was decreased (46.3-51.8, average 49.5 ± 2.2) to the same degree as in rats with diabetes for over 40 days. The area of β-cell was reduced (average 106.8 ± 2.4μ<SUP>2</SUP>). The number of α-cells showed a slight decrease (average 13.7 ± 0.63), and the area of α-cell was also slightly reduced (average 56.8 ± 1.472). <BR>7) These findings indicate that a period of at least 23 days of diabetes in both male and female rats is necessary to bring about disorders in the development of β-cells in Langerhans' islets in some of their offspring ; more than 24 days of diabetes is necessary to cause some disorder in the whole litter,
- 一般社団法人 日本内分泌学会の論文