ウサギの糖尿病期間とその子のLangerhans氏島発育障碍との関係について
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概要
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It was first reported by Prof. K. OKAMOTO and his collaborators that, the hypoplasia in the Langerhans' islets, (decrease in the number and size of the β-cells, consequently shrinkage in the size of the islet) was able to be produced in the offsprings from the adult rabbits subjected to diabetes for more than a month, and that the hypoplasia became much more intensive with continuing of the successive mating generations of the diabetic rabbits, finally resulting in the "spontaneous diabetes" in the F<SUB>4</SUB> and F<SUB>5</SUB> offsprings.<BR>The following three breeding procedures, experiment A, B and C were carried out in attempt to determine the minimum duration of diabetic state in the parent rabbits to cause maleficient effects on their offsprings, and to determine the minimum duration of the cure period (aglycosuria) to prevent the effects.<BR>(a) Experiment A : mature male and female rabbits, subjected to alloxan diabetes for 7, 14, 21, 22, 23, 26, 30 days and finally for more than two months respectively were mated to obtain the offsprings.<BR>(b) Experiment B : mature male rabbits, subjected to alloxan diabetes for 14, 21, 22, 23, 24, 25, 26, 27, 30, 35 and 70 days respectively were mated with normal females.<BR>(c) Male rabbits, subjected to alloxan diabetes for more than one month and later restored to health by administration of insulin, were mated after 2, 4, 5 and 7 days of aglycosuria respectively with healthy females.<BR>Some of the young offsprings were sacrificed on the 120th day and on the 180th day after birth, weighing more than 2.0 kg, and 2.3 kg, respectively.<BR>The number and size of the α- and β-cells in the Langerhans' islet, and the size of the islet itself were measured histometrically, and were compared with those in their healthy counterparts. The following results of the experiment could be obtained : <BR>(1) In both experiment A and B, the youngs from the parents paired after more than 26 days of diabetes, showed remarkable signs of hypoplasia in the Langerhans' islet, i.e. decrease in the number and size of the β-cells, and consequently, in the size of the islet itself.<BR>In the F<SUB>1</SUB> animals from parents, both suffered from diabetes (Exp. A), the number of the β-cells decreased by 4 to 5 from the normal state, while in the F<SUB>1</SUB> offsprings from diabetic male parents (Exp. B), the number decreased by 2 or 2.5. Thus, the degree of hypoplasia in the β-cells in Experiment B proved to be half as severe as in Experiment A.<BR>(2) When the duration of diabetes in the parent rabbits was less than 22 days, all the youngs in both Experiment A and B proved to be identical in measurement with normal rabbits.<BR>(3) In Experiment B, the young offsprings from the diabetic male parents with the duration of illness for 23 to 25 days, were divided into two categories even among the littermates : one showing no histometrical changes in the islet, and the other showing the same changes as in the F<SUB>1</SUB> offsprings from the <I>fathers</I> suffered from diabetes for a long period of time. In the Experiment A, the offsprings from the parents paired after 23 days of diabetic state were divided also into two categories even among the littermates : one being identical in measurement with the <I>F</I><SUB>1</SUB> <I>offsprings from diabetic male and female parents</I>, and the other showing the same signs of the F<SUB>1</SUB> offspring from <I>the diabetic male parents</I>.<BR>(4) In Experiment C, all the offsprings from the male parents without glycosuria for 7 days were proved to be identical in measurement with normal rabbits, while in the case of 4-5 day "cure" period (aglycosuria), young ones were divided into those which showed no signs of illness and those which showed no signs of the F<SUB>1</SUB> offsprings from <I>the diabetic fathers</I>. All the offsprings from the male parents without glycosuria for 2 days,
- 一般社団法人 日本内分泌学会の論文