視床下部の甲状腺調節因子Thyrotrophin Releasing Factor (TRF) の研究
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概要
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As to the control of the thyroid by the hypothalamus, many workers now consider it as humoral regulation. In 1955, it was found out in our department that there is a tendency in the hypothalamus to stimulate selectively the hypophysis-thyroid system both functionally and morphologically, and since then studies have been performed by us on the central system governing the thyroid. As the consequence a substance was demonstrated, which was provisionally designated as thyrotryphin releasing factor. The author extracted TRF from urine, and made studies on its purification, and the following results were obtained.<BR>(1) Crude TRF was precipitated by adding zinz sulfate and potassium ferrocyanide to urine, and extracted by using 97-80% ethanol as solvent. In this way, about 150 mg of TRF was harvested from 1000 ml of urine.<BR>(2) In electrophoresis in veronal buffer (pH 8.5), crude TRF shifted toward the cathode, and in ion exchange resin, Dowes 50 × -2, it was separated in the acid portion. It showed maximum absorption of ultraviolet ray at 240 mμ and 280 mμ.<BR>(3) The -7.8 and P<SUB>2</SUB> fraction were not protein nor lipoid. As to the development of color, it was positive only in ninhydrin and Sakaguchi reaction.<BR>(4) The P<SUB>2</SUB> fraction always stimulated the hypophysis-thyroid system, activating it significantly both histologically and functionally.<BR>(5) TRF was always contained in ninhydrin positive fraction, and one and two-dimensional paper chromatography and hydrolysis suggested that it would be peptide.
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