正常ならびに実験的先天性糖尿病ウサギの尿中17-Ketosteroids総量ならびに分劃, 17-Hydroxycorticosteroids, ウロペプシンについて
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The urinary 17-ketosteroid content, 17-ketosteroid fractionation, 17-hydroxycorticosteroid content and uropepsin excretion of 13 normal female and 16 normal male adult rabbits weighing 2000-3500g. were measured. Similar measurements were also made with 5 female and 6 male rabbits (from 50 to 360 days after birth). The results were as follows : <BR>A) Urinary 17-ketosteroids.<BR>Neutral crude extracts were prepared from urine by Drekter's method, the non-ketonic fractions therein were removed by Pincus's micro-Girard method, then colorimetry was used in general compliance with Pearson's method, with a Hitachi EPU-2 spectrophotometer.<BR>1) The mean urinary 17-ketosteroid value of normal adult rabbits was 2.25+0.14 mg/48 hours (1.96-2.47 mg/48 hours) in female rabbits and 2.41 ± 0.20 mg/48 hours (1.99-2.86 mg/48 hours) in male rabbits.<BR>2) No correlation between the quantity of urine and its 17-ketosteroid content was observable.<BR>3) The urinary 17-ketosteroid content was always higher in male than in female normal rabbits and rose stepwise in both with the number of days after birth, reacting the adult mean value 120 days after birth.<BR>B) 17-Ketosteroid fractionation.<BR>The urinary 17-ketosteroids were fracionated by adsorption chromatography on alumina into eight fractions, the method used being the microscale liquid column partition chromatography of Edwards.<BR>1) Percentage of each fraction was as follows : <BR>β-17-Ketosteroids (Fractions II and III) were 24% in normal female adults and 18% in normal male adult. Fractions IV + V were 33% in female adults and 48% in male adults. The 11-oxy-17-keto-steroids (fractions VI and VII) were 26% in females, 22% in male adult rabbits.<BR>2) There was a constant difference in the 17-ketosteroid pattern between the two sexes. Fractions IV + V were always in male than in female normal rabbits.<BR>C) 17-Hydroxycorticosteroids.<BR>The butanol extracts were prepared by Smith's modification of Reddy's method, and their Porter-Silber's colorimetric reaction was analysed using a Hitachi EPU-2 spectrophotometer.<BR>1) The mean urinary 17-hydroxycorticosteroid value of normal adult rabbits was 580.9± 100.6γ/48 hours (398-720γ/48 hours) in female rabbits and at 640.6±60.2γ/48 hours (555-830γ/48 hours) in male rabbits.<BR>2) No correlation between the quantity of urine and its 17-hydrocycorticosteroid content was observable.<BR>3) The urinary 17-hydroxycorticosteroid content was always higher in male than in female normal rabbits and rose stepwise in both with the number of days after birth, reaching the adult mean value 120-130 days after birth.<BR>D) Uropepsin.<BR>The milk coagulation activity of uropepsin was examined by a modification of West's method and the proteolytic activity by Gray's or Hirshowitz's modified hemoglobin method.<BR>1) The mean uropepsin value of normal adult rabbits was 1.92 +0.23 mg/48 hours (0.76-3.36 mg/48 hours) by the modified West method or 201 ± 76.4 unit/48 hours (98-350 unit/48 hours) by the hemoglobin method in female rabbits and 1.82±0.11 mg/48 hours (0.82-3.12 mg/48 hours) by the modified West method or 199 ±79.6 unit/48 hours (96-326 unit/48 hours) by the hemoglobin method in male rabbits.<BR>2) No correlation between the quantity of urine and its uropepsin content was observable.<BR>3) There was no difference between the sexes.<BR>4) Uropepsin excretion rose stepwise in both sexes with the number of days after birth, reaching the adult highest value 120-130 days after birth and then gradually decreased.<BR>For the past several years Prof. Okamoto and his coworkers have been investigating the influence of diabetes on the offspring of rabbits and Wistar strain rats. The offspring of diabetic animals treated continuously with alloxan in every generation showed marked regular disorders in the development of the β-cells of pancreatic islets.
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